Journal: International Journal of Molecular Sciences

Article Title: Interaction of Intestinal Bacteria with Human Rotavirus during Infection in Children

doi: 10.3390/ijms22031010

Figure Lengend Snippet: Histo-blood group antigens (HBGA)-like substances on the Ruminococcus surface detected by ELISA. Ruminococcus gauvreauii strains (DSM-19829 in purple and IPLA-NM1 in blue) and Enterobacter cloacae (positive control in light blue) were recognized by anti-A, anti-B, anti-H and anti-Lewis a antibodies, indicating that these bacteria express HBGA-like substances on their surfaces. Neg.: negative control (no antibody). Statistical significance ( p < 0.05) compared to the negative control is indicated by asterisks (*).

Article Snippet: Interestingly, the antibodies against the blood groups A and B and against the H-antigen and the Lewis a antigen resulted positive for both R. gauvreauii strains tested (DSM-19829 and IPLA-NM1) as well as for the positive control E. cloacae ATCC 13047.

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Bacteria, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Interaction of Intestinal Bacteria with Human Rotavirus during Infection in Children

doi: 10.3390/ijms22031010

Figure Lengend Snippet: Ruminococcus gauvreauii and in vitro Wa infectivity. ( A ) R. gauvreauii IPLA-NM1 cells bound to the Caco-2 monolayer. Cells and bacteria were stained with DAPI, and several Z-stack images were taken and processed, as described in the Materials and Methods section . Caco-2 nuclei are in blue, and R. gauvreauii cells are in green. ( B ) Inhibitory effect of preincubation of R. gauvreauii on Wa rotavirus infection measured as the reduction in viral genome equivalents in supernatants of Caco-2 cells after infection. Statistical significance ( p < 0.05) compared to the negative control is indicated by asterisk (*).

Article Snippet: Interestingly, the antibodies against the blood groups A and B and against the H-antigen and the Lewis a antigen resulted positive for both R. gauvreauii strains tested (DSM-19829 and IPLA-NM1) as well as for the positive control E. cloacae ATCC 13047.

Techniques: In Vitro, Infection, Bacteria, Staining, Negative Control

Journal: Microbiology Insights

Article Title: Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin

doi: 10.1177/1178636118775100

Figure Lengend Snippet: The effect of naringenin on the growth of Bifidobacterium catenulatum . Bifidobacterium catenulatum was cultured for 24 hours in the presence of increasing concentrations of naringenin. A densitometer was used to measure the McFarland units at designated time points. The * mark indicates that there was a statistically significant difference between the control and experimental groups at that time point, according to a 2-tailed, Student t test ( P < .05). (A) The 24-hour growth curve of B catenulatum supplemented with increasing concentrations of naringenin. The dotted line represents bacteria grown without naringenin. (B) The percent of control for B catenulatum cultured in each concentration of naringenin at 12 and 24 hours post inoculation.

Article Snippet: All bacterial strains were ordered as freeze-dried ampoules from Deutsche SammLung von Mikroorganismen und Zellkulturen GmbH (DSMZ) in Germany: type strain Ruminococcus gauvreauii CCRI-16110 (19829), type strain Enterococcus caccae SS-1777 (19114), and type strain Bifidobacterium catenulatum B669 (16992).

Techniques: Cell Culture, Concentration Assay

Journal: Microbiology Insights

Article Title: Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin

doi: 10.1177/1178636118775100

Figure Lengend Snippet: Genes upregulated by Bifidobacterium catenulatum in response to naringenin.

Article Snippet: All bacterial strains were ordered as freeze-dried ampoules from Deutsche SammLung von Mikroorganismen und Zellkulturen GmbH (DSMZ) in Germany: type strain Ruminococcus gauvreauii CCRI-16110 (19829), type strain Enterococcus caccae SS-1777 (19114), and type strain Bifidobacterium catenulatum B669 (16992).

Techniques: Histone Deacetylase Assay

Journal: Microbiology Insights

Article Title: Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin

doi: 10.1177/1178636118775100

Figure Lengend Snippet: Genes downregulated by Bifidobacterium catenulatum in response to naringenin.

Article Snippet: All bacterial strains were ordered as freeze-dried ampoules from Deutsche SammLung von Mikroorganismen und Zellkulturen GmbH (DSMZ) in Germany: type strain Ruminococcus gauvreauii CCRI-16110 (19829), type strain Enterococcus caccae SS-1777 (19114), and type strain Bifidobacterium catenulatum B669 (16992).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Interaction of Intestinal Bacteria with Human Rotavirus during Infection in Children

doi: 10.3390/ijms22031010

Figure Lengend Snippet: Histo-blood group antigens (HBGA)-like substances on the Ruminococcus surface detected by ELISA. Ruminococcus gauvreauii strains (DSM-19829 in purple and IPLA-NM1 in blue) and Enterobacter cloacae (positive control in light blue) were recognized by anti-A, anti-B, anti-H and anti-Lewis a antibodies, indicating that these bacteria express HBGA-like substances on their surfaces. Neg.: negative control (no antibody). Statistical significance ( p < 0.05) compared to the negative control is indicated by asterisks (*).

Article Snippet: ELISA plates (Costar) were coated with the two R. gauvreauii strains (DSM-19829 and IPLA-NM1).

Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control